NOC:Proteins and Gel-Based Proteomics


Lecture 0 - Proteins and Gel-Based Proteomics; Course Introduction


Lecture 1 - Introduction to amino acids


Lecture 2 - Introduction to proteins


Lecture 3 - Protein folding & misfolding


Lecture 4 - Protein purification techniques


Lecture 5 - Introduction to proteomics


Lecture 6 - Systems biology and proteomics


Lecture 7 - Sample preparation and pre-analytical factors


Lecture 8 - Sample preparation: Pre-analytical factors (Continued...)


Lecture 9 - Sample preparation: Protein extraction and quantification


Lecture 10 - One-dimensional electrophoresis


Lecture 11 - 2-DE: Rehydration, IEF & Equilibration


Lecture 12 - 2-DE: Second dimension, staining & destaining


Lecture 13 - 2-DE: Gel analysis


Lecture 14 - 2-DE: Applications


Lecture 15 - 2-DE: Applications (Continued...) & Challenges


Lecture 16 - 2D-DIGE: Basics


Lecture 17 - 2D-DIGE: Data analysis


Lecture 18 - 2D-DIGE: Applications


Lecture 19 - Protein identification using MALDI-TOF/TOF


Lecture 20 - Proteomics experiment data analysis & challenges