NOC:Interactomics: Protein Arrays and Label-free Biosensors


Lecture 1 - Introduction to Interactomics


Lecture 2 - An overview of label-free technologies


Lecture 3 - An overview of surface plasmon resonance (SPR)


Lecture 4 - An overview of surface plasmon resonance imaging (SPRi)


Lecture 5 - Basics of SPR: Surface chemistry


Lecture 6 - Basics of SPR: Experimental design


Lecture 7 - Protein immobilization for protein-protein interaction studies


Lecture 8 - Protein-protein interaction study: Binding analysis


Lecture 9 - Protein-protein interaction study: Kinetic analysis


Lecture 10 - Protein-small molecule interaction study: Immobilization and binding analysis


Lecture 11 - Protein-small molecule interaction study: Kinetic analysis


Lecture 12 - SPR: Interactive Session - I


Lecture 13 - SPR: Interactive Session - II


Lecture 14 - An overview of ellipsometry and interferometry techniques


Lecture 15 - An introduction to BioLayer Interferometry (BLI) and its applications in protein research


Lecture 16 - Kinetic analysis of protein-protein interaction using BLI


Lecture 17 - Label-free quantification of proteins using BLI


Lecture 18 - Diffraction-based biosensors - I


Lecture 19 - Diffraction-based biosensors - II


Lecture 20 - Nanotechniques in proteomics - I


Lecture 21 - Nanotechniques in proteomics - II


Lecture 22 - High throughputplatforms of interactomics: Protein arrays


Lecture 23 - Conventional label based detection techniques for Protein microarrays


Lecture 24 - Novel detection techniques for Protein microarrays


Lecture 25 - Recombinational cloning and its application for Protein microarrays


Lecture 26 - An introduction to Cell-free protein synthesis


Lecture 27 - Cell-free synthesis based protein microarrays: PISA and NAPPA


Lecture 28 - Cell-free synthesis based protein microarrays: MIST, DAPA and Halotag Arrays


Lecture 29 - Digging deeper into NAPPA: Basic Workflow


Lecture 30 - Digging deeper into NAPPA: Surface Chemistry, Printing and Assessment


Lecture 31 - Application of cell free expression protein microarrays in biomarker discovery


Lecture 32 - Application of cell free expression protein microarrays in immunological studies


Lecture 33 - Basics of microarray image scanning


Lecture 34 - Software for Image scanning and data processing


Lecture 35 - Microarray Data Analysis - Part I


Lecture 36 - Microarray Data Analysis - Part II


Lecture 37 - Application of protein microarray in biomarker discovery - I


Lecture 38 - Application of protein microarray in biomarker discovery - II


Lecture 39 - Systems biology and networks


Lecture 40 - Challenges in proteomics