NOC:Introduction to Proteomics


Lecture 1 - Introduction to amino acids


Lecture 2 - Introduction to proteins


Lecture 3 - Protein folding and misfolding


Lecture 4 - Introduction to proteomics


Lecture 5 - Lab session – Protein-protein interaction using label-free biosensors


Lecture 6 - Sample preparation and pre-analytical factors


Lecture 7 - Sample preparation: Pre-analytical factors (Continued...)


Lecture 8 - Sample preparation: Protein extraction and quantification


Lecture 9 - One-dimensional electrophoresis


Lecture 10 - Introduction to 2-DE


Lecture 11 - 2-DE: Second dimension, staining and destaining


Lecture 12 - 2-DE: Gel analysis


Lecture 13 - 2-DE Applications


Lecture 14 - 2-DE Applications (Continued...) and Challenges


Lecture 15 - Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR and data analysis


Lecture 16 - 2D-DIGE: Basics


Lecture 17 - 2D-DIGE: Data analysis


Lecture 18 - 2D-DIGE: Applications


Lecture 19 - Systems biology and proteomics - I


Lecture 20 - Systems biology and proteomics - II


Lecture 21 - Fundamentals of mass spectrometry


Lecture 22 - Chromatography technologies


Lecture 23 - Liquid chromatography


Lecture 24 - Mass spectrometry: Ionization sources


Lecture 25 - Mass spectrometry: Mass analyzers


Lecture 26 - MALDI sample preparation and analysis


Lecture 27 - Hybrid mass spectrometry configurations


Lecture 28 - Lab session - Demonstration of Q-TOF MS technology


Lecture 29 - In-gel and in-solution digestion


Lecture 30 - Lab session - Sample preparation: tissue sample preservation technology


Lecture 31 - Introduction to quantitative proteomics


Lecture 32 - SILAC: In vivo labeling


Lecture 33 - iTRAQ: In vitro labeling


Lecture 34 - TMT: In vitro labeling


Lecture 35 - Quantitative proteomics data analysis


Lecture 36 - Proteomics applications


Lecture 37 - Challenges in proteomics


Lecture 38 - OMICS and translational research


Lecture 39 - Lab session – Targeted proteomics using triple quadrupole mass spectrometry


Lecture 40 - Lab session – Targeted proteomics: multiple reaction monitoring