NOC:Multidimensional NMR Spectroscopy for Structural Studies of Biomolecules


Lecture 1 - Introduction to NMR spectroscopy


Lecture 2 - Energy levels in NMR spectroscopy: Quantum mechanical model and Vector model


Lecture 3 - Observing the NMR signal


Lecture 4 - Basic concepts in 1D NMR: Chemical shift and Spin-spin coupling


Lecture 5 - Basic concepts in 1D NMR: Nuclear Spin Relaxation, 1H NMR and 13C NMR


Lecture 6 - Basic concepts in 2D NMR spectroscopy


Lecture 7 - Principles of 2D correlation spectroscopy COSY


Lecture 8 - Principles of 2D Total correlation spectroscopy (TOCSY)


Lecture 9 - 2D Nuclear Overhauser Effect Spectroscopy (NOESY)


Lecture 10 - 2D NOESY and 2D ROESY


Lecture 11 - Principles of 2D Heteronuclear NMR


Lecture 12 - 2D Heteronuclear NMR: HSQC


Lecture 13 - Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part I


Lecture 14 - Heteronuclear multiple quantum coherence (HMQC) and single quantum coherence (HSQC) - Part II


Lecture 15 - 2D HSQC-TOCSY


Lecture 16 - 3D NMR Spectroscopy - Part I


Lecture 17 - 3D NMR Spectroscopy - Part II


Lecture 18 - 3D HNCA and 3D HNCO


Lecture 19 - 3D HNCACB and 3D HN(CO)CACB


Lecture 20 - Protein Backbone resonance assignment and side chain resonance assignment


Lecture 21 - Basic concepts of protein structure


Lecture 22 - Introduction to Structure Determination of Bio-Molecules by NMR


Lecture 23 - Over-expression of proteins in Bacteria


Lecture 24 - Isotope labeling of proteins for NMR studies - Part I


Lecture 25 - Isotope labeling of proteins for NMR studies - Part II


Lecture 26 - Isotope labeling of proteins for NMR studies - Part III


Lecture 27 - Isotope labeling of proteins for NMR studies - Part IV


Lecture 28 - Resonance assignments of Proteins - Part I


Lecture 29 - Resonance assignments of Proteins - Part II


Lecture 30 - Resonance assignments of Proteins - Part III


Lecture 31 - Determination of protein secondary structure from NMR data: CSI method


Lecture 32 - Determination of protein secondary structure from NMR data: J coupling based method


Lecture 33 - Determination of protein tertiary structure from NMR data - Part I


Lecture 34 - 3D NOESY HSQC


Lecture 35 - Determination of protein tertiary structure from NMR data - Part II


Lecture 36 - Understanding Protein ligand interaction by NMR : Chemical shift perturbation


Lecture 37 - Understanding Protein ligand interaction by NMR : Chemical exchange


Lecture 38 - Understanding Protein ligand interaction by NMR : T2 Filter


Lecture 39 - Understanding Protein ligand interaction by NMR : STD NMR


Lecture 40 - Understanding Protein ligand interaction by NMR : Transfer NOE NMR


Lecture 41 - Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part I


Lecture 42 - Understanding Protein ligand interaction by NMR : Diffusion ordered Spectroscopy (DOSY) - Part II